Composite

Part:BBa_K209428:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-02)

SSFYFP-hM2D-Vav


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
    Illegal PstI site found at 5560
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 239
    Illegal NheI site found at 505
    Illegal NheI site found at 3799
    Illegal PstI site found at 2984
    Illegal PstI site found at 5560
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2766
    Illegal BglII site found at 5226
    Illegal BglII site found at 7182
    Illegal BamHI site found at 706
    Illegal BamHI site found at 3593
    Illegal BamHI site found at 4303
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
    Illegal PstI site found at 5560
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 721
    Illegal PstI site found at 2984
    Illegal PstI site found at 5560
    Illegal NgoMIV site found at 5913
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2722
    Illegal BsaI.rc site found at 6090
    Illegal BsaI.rc site found at 6284
    Illegal BsaI.rc site found at 7098
    Illegal SapI site found at 4987
    Illegal SapI.rc site found at 116


Design Notes

Cloned into BBa_K209399.

Source

References